本試劑組直接測定細胞樣品中醣酵解產生的L-乳酸， 篩選糖酵解抑製劑。由於乳酸是糖解作用的產物(產生的速度快)，而且分子較小、容易通過細胞膜，在葡萄糖的代謝反應過程中，是相當重要的中間產物(intermediate)藉由乳酸的分析達到測定醣解現象能力。
Application• Direct determination of L-Lactate produced by glycolysis in cell samples. Screening of glycolysis inhibitors.
Key Features
• Fast and sensitive. Use of 5 µL sample. Linear detection range up to 10 mM L-lactate in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
• OD565nm
Samples
• Cell media
Species
• All
Procedure
• 30 min
Size
• 100 tests
Detection Limit
• NA
Shelf Life
• 6 months
More Details
• GLYCOLYSIS is one of the major metabolic pathways cells undergo to produce energy and results in the production of pyruvate. One of the eventual fates of pyruvate from this process is lactate dehydrogenase converting it to L-lactate via lactic acid fermentation allowing L-lactate to serve as an indicator of glycolysis. BioAssay Systems’ Glycolysis assay kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH which reduces a formazan dye. The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to the glycolytic rate of the cells.
