本試劑組用於定量測定脂質過氧化物（硫代巴比妥酸反應物質，TBARS）和藥物對脂質過氧化作用的影響。利用可見光設備(OD535nm)或螢光分析設備(FL560/580nm)進行試劑分析
，脂質暴露在氧中所發生的過氧化現象（peroxidation），為一種連鎖反應，由光線或金屬離子所引發造成的結果：
A. 食物酸敗（rancidity）或腐敗
B. 傷害活體組織，例如：癌症、動脈粥狀硬化、老化
C. 產生的自由基（ROO•、RO•、OH•）
A. 食物酸敗（rancidity）或腐敗
B. 傷害活體組織，例如：癌症、動脈粥狀硬化、老化
C. 產生的自由基（ROO•、RO•、OH•）
Application
• For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation.
Key Features
• Sensitive and accurate. Linear detection range: colorimetric assay 1 -30 μM, fluorometric assay 0.1 - 1.5 μM MDA.
Method
• OD535nm, or FL560/585nm
• For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation.
Key Features
• Sensitive and accurate. Linear detection range: colorimetric assay 1 -30 μM, fluorometric assay 0.1 - 1.5 μM MDA.
Method
• OD535nm, or FL560/585nm
Samples
• Serum, plasma, urine, saliva etc
Species
• All
Procedure
• 80 min
Size
• 100 tests
Detection Limit
• 0.1 μM
Shelf Life
• 6 months
More Details
• Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. BioAssay Systems’ TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (λex/em = 560nm/585nm) is directly proportional to TBARS concentration in the sample.
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